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Sangon Biotech primer premier 6 software
Primer Premier 6 Software, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/primer+6+software/0+5+primer+software/pm42298383-120-8-15
Average 86 stars, based on 1 article reviews
primer premier 6 software - by Bioz Stars, 2026-09
86/100 stars

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Article Title: Transcriptomic analysis to elucidate the molecular mechanisms underlying the growth advantage of Kluyveromyces marxianus XZ1 and the development of resistance in apple induced by XZ1
Article Snippet: The preceding study demonstrated that Kluyveromyces marxianus XZ1 (XZ1) exhibits potential as a biological agent for combating disease by P. expansum in apple fruit.. This potential is primarily attributed to its capacity for competing for resources and space, as well as activating certain genes involved in the transduction of plant hormone signals.. In this study, transcriptomic analysis was performed on the interaction system of XZ1 and apple to investigate the detail molecular mechanisms of the yeast to develop competitive advantage and the molecular mechanisms of the apple to develop defense response action at the gene expression level.

Article Title: Transcriptome analysis reveals the mechanism of internode development affecting maize stalk strength
Article Snippet: .. Gene-specific primers designed using Primer 6 software were synthesized by Sangon Biotech (Shanghai, China). .. The primer sequences are listed in Table . cDNA was reverse transcribed from total RNA using a PrimeScript RT kit (Takara, Dalian, China) and then analyzed by qRT-PCR on an ABI StepOne Plus Real-Time PCR system (Applied Biosystems, CA, USA).

Article Title: Decreased Imiquimod-Induced Psoriasis-Like Skin Inflammation in a Novel Mvd F250S/+ Knock-In Mouse Model.
Article Snippet: The mevalonate-diphosphate decarboxylase (MVD) gene, a member of the mevalonate pathway, plays a critical role in regulating the biosynthesis of cholesterol, steroid hormones, and non-steroid isoprenoids.. Previous studies have suggested that the MVD c.746 T > C mutation is a major pathogenic gene of porokeratosis (PK), an autoinflammatory keratinization disease (AIKD) with unclear pathogenesis, few effective treatments, and no suitable animal model. To investigate the function of MvdF250S/+ mutation, we developed a novel MvdF250S/+ mouse model carrying an equivalent point mutation to the most common genetic variation among Chinese PK patients (MVDF249S/+) using CRISPR/ Cas9 technology, which exhibited reduced cutaneous expression of Mvd protein.. In the absence of external stimulation, MvdF250S/+ mice did not display specific phenotypes.

Article Title: Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech. cDNAs were acquired by reverse transcription from 1 μg total RNA using PrimeScriptRT reagent Kit with gDNA Eraser (TAKARA, Tokyo, Japan) manufacturer’s protocol. qRT-PCR analysis was performed on ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Premix Ex Taq II Tli RNaseH Plus (TAKARA, Tokyo, Japan) and following the manufacturer’s protocol. ..

Article Title: Molecular cloning and gene expression of acc2 from grass carp (Ctenopharyngodon idella) and the regulation of glucose metabolism by ACCs inhibitor.
Article Snippet: Background Acetyl-CoA carboxylase (ACC) catalyzes the carboxylation of acetyl-CoA to malonyl-CoA.. Malonyl-CoA, which plays a key role in regulating glucose and lipid metabolism, is not only a substrate for fatty acid synthesis but also an inhibitor of the oxidation pathway.. ACC exists as two isoenzymes that are encoded by two different genes.

Article Title: A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech (Shanghai, China). .. The gene specific primers are listed in Supplementary Table . cDNAs were reverse transcribed from total RNA using the PrimeScript RT reagent Kit (Takara, Dalian, China).

Article Title: Nutritional and physiological responses of female and male largemouth bass (Micropterus salmoides) to different dietary starch levels
Article Snippet: In this study, we adopted a 2 × 2 factorial design incorporating different dietary starch levels and sexes to assess the impact of dietary starch on the growth performance, feeding response, and glucolipid metabolism of female and male largemouth bass.. Two isonitrogenous (49.6%) and isolipidic (10.4%) diets were formulated with 10% starch (low-starch level) and 20% starch (high-starch level).. The results indicated that male largemouth bass exhibited superior growth performance compared to females regardless of the dietary starch levels.

Synthesized:

Article Title: Transcriptomic analysis to elucidate the molecular mechanisms underlying the growth advantage of Kluyveromyces marxianus XZ1 and the development of resistance in apple induced by XZ1
Article Snippet: The preceding study demonstrated that Kluyveromyces marxianus XZ1 (XZ1) exhibits potential as a biological agent for combating disease by P. expansum in apple fruit.. This potential is primarily attributed to its capacity for competing for resources and space, as well as activating certain genes involved in the transduction of plant hormone signals.. In this study, transcriptomic analysis was performed on the interaction system of XZ1 and apple to investigate the detail molecular mechanisms of the yeast to develop competitive advantage and the molecular mechanisms of the apple to develop defense response action at the gene expression level.

Article Title: Transcriptome analysis reveals the mechanism of internode development affecting maize stalk strength
Article Snippet: .. Gene-specific primers designed using Primer 6 software were synthesized by Sangon Biotech (Shanghai, China). .. The primer sequences are listed in Table . cDNA was reverse transcribed from total RNA using a PrimeScript RT kit (Takara, Dalian, China) and then analyzed by qRT-PCR on an ABI StepOne Plus Real-Time PCR system (Applied Biosystems, CA, USA).

Article Title: Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech. cDNAs were acquired by reverse transcription from 1 μg total RNA using PrimeScriptRT reagent Kit with gDNA Eraser (TAKARA, Tokyo, Japan) manufacturer’s protocol. qRT-PCR analysis was performed on ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Premix Ex Taq II Tli RNaseH Plus (TAKARA, Tokyo, Japan) and following the manufacturer’s protocol. ..

Article Title: A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech (Shanghai, China). .. The gene specific primers are listed in Supplementary Table . cDNAs were reverse transcribed from total RNA using the PrimeScript RT reagent Kit (Takara, Dalian, China).

Article Title: Nutritional and physiological responses of female and male largemouth bass (Micropterus salmoides) to different dietary starch levels
Article Snippet: In this study, we adopted a 2 × 2 factorial design incorporating different dietary starch levels and sexes to assess the impact of dietary starch on the growth performance, feeding response, and glucolipid metabolism of female and male largemouth bass.. Two isonitrogenous (49.6%) and isolipidic (10.4%) diets were formulated with 10% starch (low-starch level) and 20% starch (high-starch level).. The results indicated that male largemouth bass exhibited superior growth performance compared to females regardless of the dietary starch levels.

Quantitative RT-PCR:

Article Title: Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech. cDNAs were acquired by reverse transcription from 1 μg total RNA using PrimeScriptRT reagent Kit with gDNA Eraser (TAKARA, Tokyo, Japan) manufacturer’s protocol. qRT-PCR analysis was performed on ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Premix Ex Taq II Tli RNaseH Plus (TAKARA, Tokyo, Japan) and following the manufacturer’s protocol. ..

Article Title: A loose endosperm structure of wheat seed produced under low nitrogen level promotes early germination by accelerating water uptake
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech (Shanghai, China). .. The gene specific primers are listed in Supplementary Table . cDNAs were reverse transcribed from total RNA using the PrimeScript RT reagent Kit (Takara, Dalian, China).

Reverse Transcription:

Article Title: Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech. cDNAs were acquired by reverse transcription from 1 μg total RNA using PrimeScriptRT reagent Kit with gDNA Eraser (TAKARA, Tokyo, Japan) manufacturer’s protocol. qRT-PCR analysis was performed on ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Premix Ex Taq II Tli RNaseH Plus (TAKARA, Tokyo, Japan) and following the manufacturer’s protocol. ..

Real-time Polymerase Chain Reaction:

Article Title: Screening of Deoxynivalenol Producing Strains and Elucidation of Possible Toxigenic Molecular Mechanism
Article Snippet: .. Primers for qRT-PCR were designed using the Primer 6 software and synthesized by Sangon Biotech. cDNAs were acquired by reverse transcription from 1 μg total RNA using PrimeScriptRT reagent Kit with gDNA Eraser (TAKARA, Tokyo, Japan) manufacturer’s protocol. qRT-PCR analysis was performed on ABI 7300 Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) using the SYBR Premix Ex Taq II Tli RNaseH Plus (TAKARA, Tokyo, Japan) and following the manufacturer’s protocol. ..



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